Assay | Gene | Primer and Probe sequences | Position | Amplicon size (bp) | Reference |
---|---|---|---|---|---|
Ao | 16S rRNA | Forward primer 5'- CTACGCATTTCACCGCTACAC -3' | 437-457 | 265 | [33] |
Reverse primer 5'- GGGGAAGAACACGGATAGGA -3' | 702-483 | ||||
TBL | 16S rRNA | Forward primer 5'- TCCTACGGGAGGCAGCAGT -3' | 331-349 | 466 | |
Reverse primer 5'- GGACTACCAGGGTATCTAATCCTGTT -3' | 797-772 | ||||
Hi | hpd | Forward primer 5’- GGTTAAATATGCCGATGGTGTTG -3’ | 822-844 | 151 | |
Reverse primer 5’- TGCATCTTTACGCACGGTGTA -3’ | 972-953 | ||||
Probe 5’Hex- TTGTGTACACTCCGT “T” GGTAAAAGAACTTGCAC -SpC6 -3’* | 928-896 | ||||
Spn | lytA | Forward primer 5'- TCTTACGCAATCTAGCAGATGAAGC -3' | 306-326 | 101 | [6] |
Reverse primer 5'- GTTGTTTGGTTGGTTATTCGTGC -3' | 406-386 | ||||
Probe 5'- [6-FAM]-TTTGCCGAAAACGCTTGATACAGGG -[TAMRA] -3' | 354-330 | ||||
Mc | copB | Forward primer 5'- GTGAGTGCCGCTTTTACAACC -3' | 50-70 | 72 | [6] |
Reverse primer 5'- TGTATCGCCTGCCAAGACAA -3' | 121-102 |